pH is a critical parameter in method development and routine enzyme assay measurement. At the ideal pH value, called optimal pH, enzymes have their highest activity. Accurate and reliable ...

For the first time, researchers have successfully used computational simulations to modify an enzyme’s structure to increase the optimum temperature of a reaction. Researchers at Uppsala University ...

This article describes a simple method to determine the optimum pH for the activity of one enzyme in a coupled enzyme assay. Decoupling of the two-step reaction is achieved by heat denaturing the ...

At the optimum temperature the amylase will break down starch very quickly. At low temperatures the amylase will break starch down slowly due to reduced kinetic energy.

Enzymes are also involved in the building up of chemical molecules elsewhere in the body. In an organism, the active site of each enzyme is a different shape. It is a perfect match to the shape of the ...