Verify that the antibody is definitely able to detect the antigen in the species by checking data sheets and reference material Potential Cause: Primary and secondary antibody mismatch Raise the ...

The western blot is a method for detecting the presence and size of specific proteins. Proteins are first separated according to size or other physical properties by gel electrophoresis, then ...

Western blotting remains a cornerstone method for the detection and quantification of proteins from complex biological samples. This technique has evolved from its early qualitative roots to a more ...

Western blot detection methods each have their own advantages and disadvantages. Choosing the right one can be a difficult decision. The basic protocol of the western blot – also referred to as the ...

In this editorial, Kenneth Oh overviews the two main normalization methods used to quantify western blots and discusses common errors to avoid. What is western blot normalization? Kenneth Oh is the ...